cst 9664s (Cell Signaling Technology Inc)
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Cst 9664s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 8985 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/caspase+3/Cleaved+Caspase-3+(Asp175)+Rabbit+mAb/pmc13019076-374-20-20
Average 99 stars, based on 8985 article reviews
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other:Article Title: Sanguinarine Induces ROS-Mediated Mitochondrial Dysfunction and Inhibits AKT/GSK3 Signaling to Potentiate Apoptotic Effects in Cutaneous T-Cell Lymphoma. Article Snippet: Cutaneous Tcell lymphoma (CTCL) is a rare nonHodgkin lymphoma with limited durable treatment options and poor overall survival, underscoring the need for new therapeutic approaches.. Sanguinarine (SNG), a natural benzophenanthridine alkaloid, has demonstrated anticancer activity in several malignancies, but its potential role in CTCL remains unexplored.. We hypothesized that SNG induces oxidative stress and mitochondrial dysfunction, leading to the inhibition of the PI3K/AKT/GSK3 pathway and apoptosis in CTCL cells. Western Blot:Article Title: Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC Article Snippet: Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). .. Twenty micrograms of protein was heat-denatured, resolved on a 4 to 20% precast polyacrylamide gel (Bio-Rad, 5671094), and analyzed by Western blotting using the primary antibodies C15ORF48 (1:1000, Novus Biologicals, NBP1-98391), vinculin (1:10,000, Abcam, ab129002), Article Title: Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC. Article Snippet: Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). .. Twenty micrograms of protein was heat- denatured, resolved on a 4 to 20% precast polyacrylamide gel (Bio- Rad, 5671094), and analyzed by Western blotting using the primary antibodies C15ORF48 (1:1000, Novus Biologicals, NBP198391), vinculin (1:10,000, Abcam, ab129002), Incubation:Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein Article Snippet: For Western blotting analysis, 30–60 μg protein lysates were separated in 8–12 % sodium dodecyl sulfate–polyacrylamide gels and transferred onto nitrocellulose membranes (#HAWP04700, Millipore, Billerica, MA, USA). .. The membranes were blocked with 5 % skim milk (#70166, Sigma-Aldrich) for 1 h and incubated overnight at 4 °C with primary antibodies against GFP (#Ab290, Abcam, Cambridge, MA, USA), Article Title: Mitochondrial permeability transition pore desensitization by a novel dispiranic derivative prevents cardiac reperfusion injury Article Snippet: .. After electrophoretic separation, proteins were transferred onto nitrocellulose membranes that were incubated overnight with the following primary antibodies: cleaved PARP (Cell signaling, 9541, 1:1000), |
