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cst 9664s  (Cell Signaling Technology Inc)


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Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc cst 9664s
    Cst 9664s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 8985 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+3/Cleaved+Caspase-3+(Asp175)+Rabbit+mAb/pmc13019076-374-20-20
    Average 99 stars, based on 8985 article reviews
    cst 9664s - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: Sanguinarine Induces ROS-Mediated Mitochondrial Dysfunction and Inhibits AKT/GSK3 Signaling to Potentiate Apoptotic Effects in Cutaneous T-Cell Lymphoma.
    Article Snippet: Cutaneous Tcell lymphoma (CTCL) is a rare nonHodgkin lymphoma with limited durable treatment options and poor overall survival, underscoring the need for new therapeutic approaches.. Sanguinarine (SNG), a natural benzophenanthridine alkaloid, has demonstrated anticancer activity in several malignancies, but its potential role in CTCL remains unexplored.. We hypothesized that SNG induces oxidative stress and mitochondrial dysfunction, leading to the inhibition of the PI3K/AKT/GSK3 pathway and apoptosis in CTCL cells.

    Western Blot:

    Article Title: Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC
    Article Snippet: Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). .. Twenty micrograms of protein was heat-denatured, resolved on a 4 to 20% precast polyacrylamide gel (Bio-Rad, 5671094), and analyzed by Western blotting using the primary antibodies C15ORF48 (1:1000, Novus Biologicals, NBP1-98391), vinculin (1:10,000, Abcam, ab129002), caspase-3 (1:1000, Cell Signaling Technology, 9662), and NDUFA4 (1:1000, Possible Missions Inc., NC1704146) and HRP-conjugated secondary antibodies (1:10,000, Thermo Fisher Scientific, 31460). .. Proteins were visualized using SuperSignal West Dura Chemiluminescent Substrate (Thermo Fisher Scientific, 34076) on a G-Box imager (Syngene, USA).

    Article Title: Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC.
    Article Snippet: Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). .. Twenty micrograms of protein was heat- denatured, resolved on a 4 to 20% precast polyacrylamide gel (Bio- Rad, 5671094), and analyzed by Western blotting using the primary antibodies C15ORF48 (1:1000, Novus Biologicals, NBP198391), vinculin (1:10,000, Abcam, ab129002), caspase- 3 (1:1000, Cell Signaling Technology, 9662), and NDUFA4 (1:1000, Possible Missions Inc., NC1704146) and HRP- conjugated secondary antibodies (1:10,000, Thermo Fisher Scientific, 31460). .. Proteins were visualized using SuperSignal West Dura Chemiluminescent Substrate (Thermo Fisher Scientific, 34076) on a G- Box imager (Syngene, USA).

    Incubation:

    Article Title: Designing an apoptosis reporter by mutagenesis-based insertion of caspase-3 cleavage motif into green fluorescence protein
    Article Snippet: For Western blotting analysis, 30–60 μg protein lysates were separated in 8–12 % sodium dodecyl sulfate–polyacrylamide gels and transferred onto nitrocellulose membranes (#HAWP04700, Millipore, Billerica, MA, USA). .. The membranes were blocked with 5 % skim milk (#70166, Sigma-Aldrich) for 1 h and incubated overnight at 4 °C with primary antibodies against GFP (#Ab290, Abcam, Cambridge, MA, USA), caspase-3 (#9662, Cell Signaling Technology, Danvers, MA, USA), cleaved caspase-3 (#9664, CST), cytochrome C (#11940, CST), PARP (#9542, CST), and GAPDH (#LF-PA0212, Abfrontier, Seoul, Korea). .. Next, the membranes were washed five times with 10 mM Tris–HCl (pH 7.5) containing 150 mM NaCl and 0.2 % Tween-20 (TBST) and incubated with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG) or anti-mouse IgG (1:5000, both from Sigma-Aldrich) for 1 h at room temperature.

    Article Title: Mitochondrial permeability transition pore desensitization by a novel dispiranic derivative prevents cardiac reperfusion injury
    Article Snippet: .. After electrophoretic separation, proteins were transferred onto nitrocellulose membranes that were incubated overnight with the following primary antibodies: cleaved PARP (Cell signaling, 9541, 1:1000), Caspase 3 (Cell signaling, 9662, 1:1000), RIP (Cell signaling, 3493, 1:1000), β-Actin (Merck, A1978, 1:5000), ATP5A (Abcam, ab14748, 1:5000). ..



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    Image Search Results


    The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

    Journal: Poultry Science

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    doi: 10.1016/j.psj.2026.106922

    Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

    Article Snippet: Caspase 3 Rabbit Ab , Abmart , T40044 , 1: 1000.

    Techniques: Expressing

    The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

    Journal: Poultry Science

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    doi: 10.1016/j.psj.2026.106922

    Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

    Article Snippet: Caspase 3 Rabbit Ab , Abmart , T40044 , 1: 1000.

    Techniques: Activity Assay

    Effect of Et MIC2 on E.tenella infection rate and Caspase 3 Activity through ITGAV.

    Journal: Poultry Science

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    doi: 10.1016/j.psj.2026.106922

    Figure Lengend Snippet: Effect of Et MIC2 on E.tenella infection rate and Caspase 3 Activity through ITGAV.

    Article Snippet: Caspase 3 Rabbit Ab , Abmart , T40044 , 1: 1000.

    Techniques: Infection, Activity Assay